Jump to main content
Jump to site search

Issue 3, 2015
Previous Article Next Article

A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

Author affiliations

Abstract

Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn2+ in lysosomes of HeLa cells, endogenous Zn2+ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy.

Graphical abstract: A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Nov 2014, accepted on 02 Jan 2015 and first published on 23 Jan 2015


Article type: Edge Article
DOI: 10.1039/C4SC03388D
Citation: Chem. Sci., 2015,6, 1944-1948
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

    P. Rivera-Fuentes, A. T. Wrobel, M. L. Zastrow, M. Khan, J. Georgiou, T. T. Luyben, J. C. Roder, K. Okamoto and S. J. Lippard, Chem. Sci., 2015, 6, 1944
    DOI: 10.1039/C4SC03388D

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements