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Issue 3, 2015
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A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

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Abstract

Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn2+ in lysosomes of HeLa cells, endogenous Zn2+ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy.

Graphical abstract: A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

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Publication details

The article was received on 04 Nov 2014, accepted on 02 Jan 2015 and first published on 23 Jan 2015


Article type: Edge Article
DOI: 10.1039/C4SC03388D
Citation: Chem. Sci., 2015,6, 1944-1948
  • Open access: Creative Commons BY-NC license
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    A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

    P. Rivera-Fuentes, A. T. Wrobel, M. L. Zastrow, M. Khan, J. Georgiou, T. T. Luyben, J. C. Roder, K. Okamoto and S. J. Lippard, Chem. Sci., 2015, 6, 1944
    DOI: 10.1039/C4SC03388D

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