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Issue 1, 2015
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Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

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Abstract

The cytoprotection of individual living cells under in vitro and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate Saccharomyces cerevisiae in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure acquires multiple resistance against lytic enzyme, desiccation, and UV-C irradiation. In addition to the UV-C filtering effect of the double-layered shell, the biochemical responses of the encapsulated yeast are suggested to contribute to the observed UV-C tolerance. This work offers a chemical tool for cytoprotecting individual living cells under multiple stresses and also for studying biochemical behavior at the cellular level.

Graphical abstract: Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

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Publication details

The article was received on 10 Sep 2014, accepted on 30 Sep 2014 and first published on 01 Oct 2014


Article type: Edge Article
DOI: 10.1039/C4SC02789B
Citation: Chem. Sci., 2015,6, 203-208
  • Open access: Creative Commons BY-NC license
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    Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

    D. Hong, H. Lee, E. H. Ko, J. Lee, H. Cho, M. Park, S. H. Yang and I. S. Choi, Chem. Sci., 2015, 6, 203
    DOI: 10.1039/C4SC02789B

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