Issue 22, 2015
From the journal:

RSC Advances

Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry†

Hideaki Fujita,ab   Tsuyoshi Esaki,c   Tsutomu Masujima,c   Akitsu Hotta,de   Soo Hyeon Kim,f   Hiroyuki Nojif  and  Tomonobu M. Watanabe*abeg  

* Corresponding authors

a Immunology Frontier Research Center, Osaka University, 1-3 Yamadaoka, Suita-shi, OSAKA, Japan

b Laboratory for Comprehensive Bioimaging, Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita-shi, OSAKA, Japan
E-mail: tomowatanabe@riken.jp
Fax: +81-6-6849-4425
Tel: +81-6-6849-4426

c Laboratory for single cell mass spectrometry, Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita-shi, OSAKA, Japan

d Centar for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, Japan

e PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, Japan

f Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan

g Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka, Japan

Abstract

Secretomics, the comprehensive study of cell releasates, offers a non-invasive approach to understanding cell heterogeneity. We here propose single cell small molecule secretomics using micro-droplet arrays and mass spectrometry as a new repertoire of omics technologies. The present method revealed the heterogeneity of secreted small molecules from individual single cells without the use of any invasive processes.

Graphical abstract: Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C4RA12021C
Article type
Communication
Submitted
09 Oct 2014
Accepted
29 Jan 2015
First published
30 Jan 2015

RSC Adv., 2015,5, 16968-16971

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Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry

H. Fujita, T. Esaki, T. Masujima, A. Hotta, S. H. Kim, H. Noji and T. M. Watanabe, RSC Adv., 2015, 5, 16968 DOI: 10.1039/C4RA12021C

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