Issue 106, 2015

Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus

Abstract

Herein, we construct a novel cell-based fluorescence response screening system based on a single insertion of circularly permuted yellow fluorescent protein (cpYFP) to reflect the protein conformational change caused by MgrA–small molecules interaction. And this screening platform was applied for screening of Chinese herb extracts with two extracts identified from 351.

Graphical abstract: Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus

Supplementary files

Article information

Article type
Communication
Submitted
16 Jun 2015
Accepted
07 Oct 2015
First published
08 Oct 2015

RSC Adv., 2015,5, 87216-87220

Author version available

Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus

Y. Wang, H. Zhang, Q. Zhang, Y. Liang, L. Ma, H. Tan, Y. Lao, H. Xu and Z. Li, RSC Adv., 2015, 5, 87216 DOI: 10.1039/C5RA11455A

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