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Issue 73, 2015
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Capillary isotachophoresis for separation of silver nanoparticles according to size

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Abstract

Capillary isotachophoresis (ITP) was used for the separation of Ag nanoparticles according to their size. For this purpose, ethanol–water dispersions of Ag nanoparticles stabilized by gelatin were prepared. The ITP separations were performed in a column-coupling system filled with two electrolytes with a pH of leading electrolytes of 7.1 (system I – LE: 10 mM HNO3, ε-aminocaproic acid, TE: 10 mM caproic acid) and 4.5 (system II – LE: 10 mM HNO3, imidazole, TE: 5 mM 2-(N-morpholino)ethanesulfonic acid). In both electrolyte systems the four main peak-mode zones of Ag nanoparticles migrating at zone boundaries were identified, however, the better separation was achieved by the system II. The Ag nanoparticle dispersions were also examined by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The zeta potential and thickness of gelatin double layers adsorbed on the nanoparticles were found to depend on pH. The TEM analysis revealed four size fractions of 4 nm, 10 nm, 16 nm and 22 nm, which correspond to the zones separated by ITP. Other migration zones of the electrolyte systems, such as impurities and/or products of the nanoparticle synthesis, served as spacers and separated the peak zones of the Ag nanoparticles.

Graphical abstract: Capillary isotachophoresis for separation of silver nanoparticles according to size

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Publication details

The article was received on 05 Jun 2015, accepted on 30 Jun 2015 and first published on 01 Jul 2015


Article type: Paper
DOI: 10.1039/C5RA10676A
Author version available: Download Author version (PDF)
Citation: RSC Adv., 2015,5, 59131-59136
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    Capillary isotachophoresis for separation of silver nanoparticles according to size

    P. Praus, M. Turicová, P. Suchomel and L. Kvítek, RSC Adv., 2015, 5, 59131
    DOI: 10.1039/C5RA10676A

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