Issue 62, 2015

Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

Abstract

In this work, thin films consisting of electrically conducting polyaniline (PANI) and poly(2-acrylamido-2-methyl-1-propanesulfonate) (PAMPSA) have been used as cell culture substrates for embryonic stem cells (ESC) and embryoid bodies (EMB). The PANI–PAMPSA films having fibrillar morphology were electrochemically polymerized in a single-step by cyclic voltammetry from an aqueous solution containing aniline and PAMPSA. UV-visible spectroscopy showed that the PANI films were electrically conducting still at pH 10. This makes them suitable for tissue engineering applications operating at physiological pH, in contrast to the commonly used PANI hydrochloride films which loose their electrical conductivity at pH ≥ 4. Our results reveal that the PANI–PAMPSA films allow only for limited ESC adhesion and growth. The inhibition of the EMB growth and adhesion on the PANI–PAMPSA surface in serum-free medium indicates that it can be used as a cell-selective substrate for the growth of only some specific differentiated EMB cell types.

Graphical abstract: Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

Article information

Article type
Paper
Submitted
24 Apr 2015
Accepted
29 May 2015
First published
29 May 2015
This article is Open Access
Creative Commons BY license

RSC Adv., 2015,5, 50328-50335

Author version available

Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

P. Bober, P. Humpolíček, J. Pacherník, J. Stejskal and T. Lindfors, RSC Adv., 2015, 5, 50328 DOI: 10.1039/C5RA07504A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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