Electrooxidation and determination of perphenazine on a graphene oxide nanosheet-modified electrode

Abstract

The electrochemical behavior of perphenazine was investigated on a graphene oxide nanosheet-modified glassy carbon electrode in a phosphate buffer solution at pH 7.4. Cyclic voltammetry was employed to study the drug electrooxidation process. Perphenazine was electrooxidized on the modified electrode surface at lower potentials with a higher rate through an irreversible process. The kinetic parameters of the electrooxidation reaction, including the standard rate constant as well as diffusion and charge transfer coefficients were obtained. Amperometric and differential pulse voltammetric procedures were developed for the determination of perphenazine. Linear dynamic ranges of 0.8–8.0 and 0.7–7.0 mmol L⁻¹ with calibration sensitivities of 35.23 and 36.54 mA mol⁻¹ L cm⁻² and detection limits of 46.6 and 38.4 μmol L⁻¹ were obtained using amperometry and differential pulse voltammetry, respectively. The amperometric method was applied in the analysis of perphenazine tablets, and the applicability of the method for the direct assays of spiked human serum, urine and breast milk fluids was investigated.