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Issue 5, 2015
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Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells

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Abstract

Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE (Soluble NSF Attachment Protein Receptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (∼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.

Graphical abstract: Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells

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Publication details

The article was received on 14 Nov 2014, accepted on 23 Mar 2015 and first published on 24 Mar 2015


Article type: Paper
DOI: 10.1039/C4PP00423J
Citation: Photochem. Photobiol. Sci., 2015,14, 1005-1012
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    Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells

    Y. Antoku, P. Dedecker, P. S. Pinheiro, T. Vosch and J. B. Sørensen, Photochem. Photobiol. Sci., 2015, 14, 1005
    DOI: 10.1039/C4PP00423J

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