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Issue 7, 2015
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Rapid and scalable assembly of firefly luciferase substrates

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Abstract

Bioluminescence imaging with luciferase–luciferin pairs is a popular method for visualizing biological processes in vivo. Unfortunately, most luciferins are difficult to access and remain prohibitively expensive for some imaging applications. Here we report cost-effective and efficient syntheses of D-luciferin and 6′-aminoluciferin, two widely used bioluminescent substrates. Our approach employs inexpensive anilines and Appel's salt to generate the luciferin cores in a single pot. Additionally, the syntheses are scalable and can provide multi-gram quantities of both substrates. The streamlined production and improved accessibility of luciferin reagents will bolster in vivo imaging efforts.

Graphical abstract: Rapid and scalable assembly of firefly luciferase substrates

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Publication details

The article was received on 03 Dec 2014, accepted on 09 Dec 2014 and first published on 17 Dec 2014


Article type: Paper
DOI: 10.1039/C4OB02529F
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2015,13, 2117-2121
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    Rapid and scalable assembly of firefly luciferase substrates

    D. C. McCutcheon, W. B. Porterfield and J. A. Prescher, Org. Biomol. Chem., 2015, 13, 2117
    DOI: 10.1039/C4OB02529F

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