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Issue 41, 2015
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Quantitative nanoscale electrostatics of viruses

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Abstract

Electrostatics is one of the fundamental driving forces of the interaction between biomolecules in solution. In particular, the recognition events between viruses and host cells are dominated by both specific and non-specific interactions and the electric charge of viral particles determines the electrostatic force component of the latter. Here we probe the charge of individual viruses in liquid milieu by measuring the electrostatic force between a viral particle and the Atomic Force Microscope tip. The force spectroscopy data of co-adsorbed ϕ29 bacteriophage proheads and mature virions, adenovirus and minute virus of mice capsids is utilized for obtaining the corresponding density of charge for each virus. The systematic differences of the density of charge between the viral particles are consistent with the theoretical predictions obtained from X-ray structural data. Our results show that the density of charge is a distinguishing characteristic of each virus, depending crucially on the nature of the viral capsid and the presence/absence of the genetic material.

Graphical abstract: Quantitative nanoscale electrostatics of viruses

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Publication details

The article was received on 26 Jun 2015, accepted on 16 Jul 2015 and first published on 22 Jul 2015


Article type: Paper
DOI: 10.1039/C5NR04274G
Author version available: Download Author version (PDF)
Citation: Nanoscale, 2015,7, 17289-17298
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    Quantitative nanoscale electrostatics of viruses

    M. Hernando-Pérez, A. X. Cartagena-Rivera, A. Lošdorfer Božič, P. J. P. Carrillo, C. San Martín, M. G. Mateu, A. Raman, R. Podgornik and P. J. de Pablo, Nanoscale, 2015, 7, 17289
    DOI: 10.1039/C5NR04274G

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