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Issue 46, 2015
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Fast and background-free three-dimensional (3D) live-cell imaging with lanthanide-doped upconverting nanoparticles

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Abstract

We report on the development of a three-dimensional (3D) live-cell imaging technique with high spatiotemporal resolution using lanthanide-doped upconverting nanoparticles (UCNPs). It employs the sectioning capability of confocal microscopy except that the two-dimensional (2D) section images are acquired by wide-field epi-fluorescence microscopy. Although epi-fluorescence images are contaminated with the out-of-focus background in general, the near-infrared (NIR) excitation used for the excitation of UCNPs does not generate any autofluorescence, which helps to lower the background. Moreover, the image blurring due to defocusing was naturally eliminated in the image reconstruction process. The 3D images were used to investigate the cellular dynamics such as nuclear uptake and single-particle tracking that require 3D description.

Graphical abstract: Fast and background-free three-dimensional (3D) live-cell imaging with lanthanide-doped upconverting nanoparticles

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Publication details

The article was received on 28 Aug 2015, accepted on 25 Oct 2015 and first published on 28 Oct 2015


Article type: Communication
DOI: 10.1039/C5NR05875A
Citation: Nanoscale, 2015,7, 19397-19402
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    Fast and background-free three-dimensional (3D) live-cell imaging with lanthanide-doped upconverting nanoparticles

    H. L. Jo, Y. H. Song, J. Park, E. Jo, Y. Goh, K. Shin, M. Kim and K. T. Lee, Nanoscale, 2015, 7, 19397
    DOI: 10.1039/C5NR05875A

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