Jump to main content
Jump to site search

Issue 42, 2015
Previous Article Next Article

Cell-specific optoporation with near-infrared ultrafast laser and functionalized gold nanoparticles

Author affiliations

Abstract

Selective targeting of diseased cells can increase therapeutic efficacy and limit off-target adverse effects. We developed a new tool to selectively perforate living cells with functionalized gold nanoparticles (AuNPs) and near-infrared (NIR) femtosecond (fs) laser. The receptor CD44 strongly expressed by cancer stem cells was used as a model for selective targeting. Citrate-capped AuNPs (100 nm in diameter) functionalized with 0.01 orthopyridyl-disulfide-poly(ethylene glycol) (5 kDa)-N-hydroxysuccinimide (OPSS-PEG-NHS) conjugated to monoclonal antibodies per nm2 and 5 μM HS-PEG (5 kDa) were colloidally stable in cell culture medium containing serum proteins. These AuNPs attached mostly as single particles 115 times more to targeted CD44+ MDA-MB-231 and CD44+ ARPE-19 cells than to non-targeted CD44 661W cells. Optimally functionalized AuNPs enhanced the fs laser (800 nm, 80–100 mJ cm−2 at 250 Hz or 60–80 mJ cm−2 at 500 Hz) to selectively perforate targeted cells without affecting surrounding non-targeted cells in co-culture. This novel highly versatile treatment paradigm can be adapted to target and perforate other cell populations by adapting to desired biomarkers. Since living biological tissues absorb energy very weakly in the NIR range, the developed non-invasive tool may provide a safe, cost-effective clinically relevant approach to ablate pathologically deregulated cells and limit complications associated with surgical interventions.

Graphical abstract: Cell-specific optoporation with near-infrared ultrafast laser and functionalized gold nanoparticles

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Aug 2015, accepted on 19 Sep 2015 and first published on 05 Oct 2015


Article type: Paper
DOI: 10.1039/C5NR05650K
Citation: Nanoscale, 2015,7, 17836-17847
  •   Request permissions

    Cell-specific optoporation with near-infrared ultrafast laser and functionalized gold nanoparticles

    E. Bergeron, C. Boutopoulos, R. Martel, A. Torres, C. Rodriguez, J. Niskanen, J. Lebrun, F. M. Winnik, P. Sapieha and M. Meunier, Nanoscale, 2015, 7, 17836
    DOI: 10.1039/C5NR05650K

Search articles by author

Spotlight

Advertisements