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Issue 42, 2015
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Determination of the structure and morphology of gold nanoparticle–HSA protein complexes

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Abstract

We propose a simple method to determine the structure and morphology of nanoparticle protein complexes. By combining a separation method with online size measurements, density measurements and circular dichroism, we could identify the number of proteins bound to each nanoparticle and their secondary structure changes in the complex. This method provides much-needed experimental information on the interaction of proteins with nanoparticles and on the behavior of nanoparticles in biological systems.

Graphical abstract: Determination of the structure and morphology of gold nanoparticle–HSA protein complexes

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Publication details

The article was received on 31 Jul 2015, accepted on 27 Sep 2015 and first published on 14 Oct 2015


Article type: Communication
DOI: 10.1039/C5NR05147A
Citation: Nanoscale, 2015,7, 17653-17657
  • Open access: Creative Commons BY-NC license
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    Determination of the structure and morphology of gold nanoparticle–HSA protein complexes

    R. Capomaccio, I. Ojea Jimenez, P. Colpo, D. Gilliland, G. Ceccone, F. Rossi and L. Calzolai, Nanoscale, 2015, 7, 17653
    DOI: 10.1039/C5NR05147A

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