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Issue 32, 2015
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DNA nanopore translocation in glutamate solutions

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Abstract

Nanopore experiments have traditionally been carried out with chloride-based solutions. Here we introduce silver/silver-glutamate-based electrochemistry as an alternative, and study the viscosity, conductivity, and nanopore translocation characteristics of potassium-, sodium-, and lithium-glutamate solutions. We show that it has a linear response at typical voltages and can be used to detect DNA translocations through a nanopore. The glutamate anion also acts as a redox-capable thickening agent, with high-viscosity solutions capable of slowing down the DNA translocation process by up to 11 times, with a corresponding 7 time reduction in signal. These results demonstrate that glutamate can replace chloride as the primary anion in nanopore resistive pulse sensing.

Graphical abstract: DNA nanopore translocation in glutamate solutions

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Publication details

The article was received on 29 Apr 2015, accepted on 05 Jul 2015 and first published on 10 Jul 2015


Article type: Paper
DOI: 10.1039/C5NR02793D
Citation: Nanoscale, 2015,7, 13605-13609
  • Open access: Creative Commons BY license
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    DNA nanopore translocation in glutamate solutions

    C. Plesa, N. van Loo and C. Dekker, Nanoscale, 2015, 7, 13605
    DOI: 10.1039/C5NR02793D

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