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Issue 17, 2015
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Charged group surface accessibility determines micelleplexes formation and cellular interaction

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Abstract

Micelleplexes are a class of nucleic acid carriers that have gained acceptance due to their size, stability, and ability to synergistically carry small molecules. MicroRNAs (miRNAs) are small non-coding RNA gene regulator that is consists of 19–22 nucleotides. Altered expression of miRNAs plays an important role in many human diseases. Using a model 22-nucleotide miRNA sequence, we investigated the interaction between charged groups on the micelle surface and miRNA. The model micelle system was formed from methoxy-poly(ethylene glycol)-b-poly(lactide) (mPEG-PLA) mixed with methoxy-poly(ethylene glycol)-b-poly(lactide)-b-oligoarginine (mPEG-PLA-Rx, x = 8 or 15). Surface properties of the micelles were varied by controlling the oligoarginine block length and conjugation density. Micelles were observed to have a core–shell conformation in the aqueous environment where the PLA block constituted the hydrophobic core, mPEG and oligoarginine formed a hydrophilic corona. Significantly different thermodynamic behaviors were observed during the interaction of single stranded miRNA with micelles of different surface properties, and the resulting micelleplexes mediated substantial cellular association. Depending upon the oligoarginine length and density, micelles exhibited miRNA loading capacity directly related to the presentation of charged groups on the surface. The effect of charged group accessibility of cationic micelle on micelleplex properties provides guidance on future miRNA delivery system design.

Graphical abstract: Charged group surface accessibility determines micelleplexes formation and cellular interaction

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Publication details

The article was received on 07 Jan 2015, accepted on 27 Mar 2015 and first published on 01 Apr 2015


Article type: Communication
DOI: 10.1039/C5NR00095E
Author version available: Download Author version (PDF)
Citation: Nanoscale, 2015,7, 7559-7564
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    Charged group surface accessibility determines micelleplexes formation and cellular interaction

    Y. Zhang, Y. Liu, S. Sen, P. Král and R. A. Gemeinhart, Nanoscale, 2015, 7, 7559
    DOI: 10.1039/C5NR00095E

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