Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium

Fatai Adigun Aborode; Andrea Raab; Simon Foster; Enzo Lombi; William Maher; Eva M. Krupp; Joerg Feldmann

doi:10.1039/C5MT00025D

Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium

Fatai Adigun Aborode,^a Andrea Raab,^a Simon Foster,^b Enzo Lombi,^c William Maher,^b Eva M. Krupp^a and Joerg Feldmann*^a

Author affiliations

* Corresponding authors

^a TESLA (Trace Element Speciation Laboratory) Department of Chemistry, Meston Walk, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK
E-mail: j.feldmann@abdn.ac.uk, faborode@hotmail.com

^b University of Canberra, Ecochemistry Laboratory, Institute for Applied Ecology, Australia

^c Centre for Environmental Risk Assessment and Remediation, University of South Australia, Building X, Mawson Lakes Campus, Mawson Lakes, Australia

Abstract

Three month old Thunbergia alata were exposed for 13 days to 10 μM selenite to determine the biotransformation of selenite in their roots. Selenium in formic acid extracts (80 ± 3%) was present as selenopeptides with Se–S bonds and selenium–PC complexes (selenocysteinyl-2-3-dihydroxypropionyl-glutathione, seleno-phytochelatin2, seleno-di-glutathione). An analytical method using HPLC-ICPMS to detect and quantify elemental selenium in roots of T. alata plants using sodium sulfite to quantitatively transform elemental selenium to selenosulfate was also developed. Elemental selenium was determined as 18 ± 4% of the total selenium in the roots which was equivalent to the selenium not extracted using formic acid extraction. The results are in an agreement with the XAS measurements of the exposed roots which showed no occurrence of selenite or selenate but a mixture of selenocysteine and elemental selenium.

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Publication details

The article was received on 26 Jan 2015, accepted on 26 Feb 2015 and first published on 26 Feb 2015

Article type: Paper

DOI: 10.1039/C5MT00025D

Author version available: Download Author version (PDF)

Citation: Metallomics, 2015,7, 1056-1066

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Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium

F. A. Aborode, A. Raab, S. Foster, E. Lombi, W. Maher, E. M. Krupp and J. Feldmann, Metallomics, 2015, 7, 1056
DOI: 10.1039/C5MT00025D
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Metallomics

Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium

Abstract

Supplementary files

Publication details

Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium

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