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Issue 2, 2015
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High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

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Abstract

Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.

Graphical abstract: High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

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Publication details

The article was received on 20 Sep 2014, accepted on 18 Nov 2014 and first published on 18 Nov 2014


Article type: Communication
DOI: 10.1039/C4MB00556B
Citation: Mol. BioSyst., 2015,11, 361-365
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    High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

    N. Takemori, A. Takemori, K. Matsuoka, R. Morishita, N. Matsushita, M. Aoshima, H. Takeda, T. Sawasaki, Y. Endo and S. Higashiyama, Mol. BioSyst., 2015, 11, 361
    DOI: 10.1039/C4MB00556B

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