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Issue 14, 2015
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A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

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Abstract

We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.

Graphical abstract: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

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Publication details

The article was received on 18 Apr 2015, accepted on 27 May 2015 and first published on 28 May 2015


Article type: Communication
DOI: 10.1039/C5LC00443H
Citation: Lab Chip, 2015,15, 2918-2922
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    A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

    N. J. Mozdzierz, K. R. Love, K. S. Lee, H. L. T. Lee, K. A. Shah, R. J. Ram and J. C. Love, Lab Chip, 2015, 15, 2918
    DOI: 10.1039/C5LC00443H

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