Issue 14, 2015
From the journal:

Lab on a Chip

A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production†

Nicholas J. Mozdzierz, ORCID logo ab   Kerry R. Love,bc   Kevin S. Lee,d   Harry L. T. Lee,d   Kartik A. Shah,b   Rajeev J. Ramce  and  J. Christopher Love*abc  

* Corresponding authors

a Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
E-mail: clove@mit.edu

b The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

c MIT Center for Biomedical Innovation, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

d Pharyx, Inc., Woburn, MA 01801, USA

e Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA

Abstract

We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.

Graphical abstract: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5LC00443H
Article type
Communication
Submitted
18 Apr 2015
Accepted
27 May 2015
First published
28 May 2015

Lab Chip, 2015,15, 2918-2922

Permissions

Request permissions

A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

N. J. Mozdzierz, K. R. Love, K. S. Lee, H. L. T. Lee, K. A. Shah, R. J. Ram and J. C. Love, Lab Chip, 2015, 15, 2918 DOI: 10.1039/C5LC00443H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements