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Issue 23, 2015
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A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

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Abstract

An electrochemical device, which consists of electrode arrays, nanocavities, and microwells, was developed for multi-electrochemical detection with high sensitivity. A local redox cycling-based electrochemical (LRC-EC) system was used for multi-electrochemical detection and signal amplification. The LRC-EC system consists of n2 sensors with only 2n bonding pads for external connection. The nanocavities fabricated in the sensor microwells enable significant improvement of the signal amplification compared with the previous devices we have developed. The present device was successfully applied for evaluation of embryoid bodies (EBs) from embryonic stem (ES) cells via electrochemical measurements of alkaline phosphatase (ALP) activity in the EBs. In addition, the EBs were successfully trapped in the sensor microwells of the device using dielectrophoresis (DEP) manipulation, which led to high-throughput cell analysis. This device is considered to be useful for multi-electrochemical detection and imaging for bioassays including cell analysis.

Graphical abstract: A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

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Publication details

The article was received on 25 Aug 2015, accepted on 06 Oct 2015 and first published on 06 Oct 2015


Article type: Paper
DOI: 10.1039/C5LC01016K
Citation: Lab Chip, 2015,15, 4404-4414
  • Open access: Creative Commons BY license
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    A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

    Y. Kanno, K. Ino, H. Shiku and T. Matsue, Lab Chip, 2015, 15, 4404
    DOI: 10.1039/C5LC01016K

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