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Issue 6, 2015
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Optofluidic ultrahigh-throughput detection of fluorescent drops

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Abstract

This paper describes an optofluidic droplet interrogation device capable of counting fluorescent drops at a throughput of 254 000 drops per second. To our knowledge, this rate is the highest interrogation rate published thus far. Our device consists of 16 parallel microfluidic channels bonded directly to a filter-coated two-dimensional Complementary Metal-Oxide-Semiconductor (CMOS) sensor array. Fluorescence signals emitted from the drops are collected by the sensor that forms the bottom of the channel. The proximity of the drops to the sensor facilitates efficient collection of fluorescence emission from the drops, and overcomes the trade-off between light collection efficiency and field of view in conventional microscopy. The interrogation rate of our device is currently limited by the acquisition speed of CMOS sensor, and is expected to increase further as high-speed sensors become increasingly available.

Graphical abstract: Optofluidic ultrahigh-throughput detection of fluorescent drops

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Publication details

The article was received on 15 Dec 2014, accepted on 08 Jan 2015 and first published on 08 Jan 2015


Article type: Paper
DOI: 10.1039/C4LC01465K
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Citation: Lab Chip, 2015,15, 1417-1423
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    Optofluidic ultrahigh-throughput detection of fluorescent drops

    M. Kim, M. Pan, Y. Gai, S. Pang, C. Han, C. Yang and S. K. Y. Tang, Lab Chip, 2015, 15, 1417
    DOI: 10.1039/C4LC01465K

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