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Issue 5, 2015
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A system for ω-transaminase mediated (R)-amination using L-alanine as an amine donor

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Abstract

Chiral amines are important building blocks for fine chemicals and pharmaceuticals. Consequently, various biocatalytic routes in particular using ω-transaminases (ω-TAs) have been developed recently. Although catalysts for the synthesis of both enantiomers are available, the application of alanine dependent (R)-selective ω-TAs is less favourable due to the requirement of the more expensive D-alanine as an amine donor. Here we describe an efficient method for (R)-amination using ω-TAs in combination with an alanine racemase (AlaR). In this case, the readily available L-alanine can be used as an amine donor leading to improved atom efficiency and significantly reduced costs.

Graphical abstract: A system for ω-transaminase mediated (R)-amination using l-alanine as an amine donor

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Publication details

The article was received on 01 Dec 2014, accepted on 10 Mar 2015 and first published on 19 Mar 2015


Article type: Paper
DOI: 10.1039/C4GC02363C
Citation: Green Chem., 2015,17, 2952-2958
  • Open access: Creative Commons BY license
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    A system for ω-transaminase mediated (R)-amination using L-alanine as an amine donor

    N. Richter, J. E. Farnberger, D. Pressnitz, H. Lechner, F. Zepeck and W. Kroutil, Green Chem., 2015, 17, 2952
    DOI: 10.1039/C4GC02363C

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