Jump to main content
Jump to site search

Issue 6, 2015
Previous Article Next Article

Lentinula edodes-derived polysaccharide enhances systemic and mucosal immunity by spatial modulation of intestinal gene expression in mice

Author affiliations

Abstract

Mushroom polysaccharides have been reported to possess significant biological activities. However, their molecular mechanisms are not fully elucidated. In this study, the immunostimulating activity of a newly purified heteropolysaccharide L2 from Lentinula edodes is evaluated in Caco-2 cells and a Caco-2/RAW264.7 co-culture system, as well as in mice. Subsequently, the customized RT-PCR array containing 112 genes is employed to investigate the effects of L2 on gene expressions in the small intestine, cecum and colon. The results show that L2 significantly enhances immune responses by differentially affecting the gene expressions of small intestine, cecum and colon, in which 55, 26 and 25 genes are markedly changed, respectively. In particular, 3 core regulation networks are identified for various parts of gut. These data demonstrate the potential of L2 as a potent immune stimulator and for the first time provide a detailed landscape of tissue-specific gene expressions and core regulation networks in response to L. edodes-derived heteropolysaccharide treatment.

Graphical abstract: Lentinula edodes-derived polysaccharide enhances systemic and mucosal immunity by spatial modulation of intestinal gene expression in mice

Back to tab navigation

Publication details

The article was received on 25 Dec 2014, accepted on 18 May 2015 and first published on 18 May 2015


Article type: Paper
DOI: 10.1039/C4FO01192A
Citation: Food Funct., 2015,6, 2068-2080
  •   Request permissions

    Lentinula edodes-derived polysaccharide enhances systemic and mucosal immunity by spatial modulation of intestinal gene expression in mice

    X. Xu, J. Yang, Z. Luo and X. Zhang, Food Funct., 2015, 6, 2068
    DOI: 10.1039/C4FO01192A

Search articles by author

Spotlight

Advertisements