Issue 9, 2015
From the journal:

Dalton Transactions

The phenanthroimidazole-based dizinc(ii) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing†

Sellamuthu Anbu,*a   Subban Kamalraj,b   Anup Paul,a   Chelliah Jayabaskaranb  and  Armando J. L. Pombeiro*a  

* Corresponding authors

a Centro de Química Estrutural, Complexo I, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
E-mail: bioinorg_anbu@yahoo.com, pombeiro@tecnico.ulisboa.pt

b Department of Biochemistry, Indian Institute of Science, Bangalore-560 012, India

Abstract

A highly selective and sensitive phenanthroimidazole tagged Mannich base type dizinc(II) fluorescent probe (R-Zn2+) has been developed for the pyrophosphate ion (PPi) with a very low limit of detection (LOD) of 0.25 ppm; this also assesses PPi from DNA polymerization chain reaction (PCR).

Graphical abstract: The phenanthroimidazole-based dizinc(ii) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing

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Article information

DOI
https://doi.org/10.1039/C4DT03590A
Article type
Communication
Submitted
22 Nov 2014
Accepted
13 Jan 2015
First published
15 Jan 2015

Dalton Trans., 2015,44, 3930-3933

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The phenanthroimidazole-based dizinc(II) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing

S. Anbu, S. Kamalraj, A. Paul, C. Jayabaskaran and A. J. L. Pombeiro, Dalton Trans., 2015, 44, 3930 DOI: 10.1039/C4DT03590A

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