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Issue 17, 2015
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Imaging metals in biology: balancing sensitivity, selectivity and spatial resolution

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Abstract

Metal biochemistry drives a diverse range of cellular processes associated with development, health and disease. Determining metal distribution, concentration and flux defines our understanding of these fundamental processes. A comprehensive analysis of biological systems requires a balance of analytical techniques that inform on metal quantity (sensitivity), chemical state (selectivity) and location (spatial resolution) with a high degree of certainty. A number of approaches are available for imaging metals from whole tissues down to subcellular organelles, as well as mapping metal turnover, protein association and redox state within these structures. Technological advances in micro- and nano-scale imaging are striving to achieve multi-dimensional and in vivo measures of metals while maintaining the native biochemical environment and physiological state. This Tutorial Review discusses state-of-the-art imaging technology as a guide to obtaining novel insight into the biology of metals, with sensitivity, selectivity and spatial resolution in focus.

Graphical abstract: Imaging metals in biology: balancing sensitivity, selectivity and spatial resolution

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Publication details

The article was received on 22 Jan 2015 and first published on 06 Jul 2015


Article type: Tutorial Review
DOI: 10.1039/C5CS00055F
Citation: Chem. Soc. Rev., 2015,44, 5941-5958
  • Open access: Creative Commons BY license
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    Imaging metals in biology: balancing sensitivity, selectivity and spatial resolution

    D. J. Hare, E. J. New, M. D. de Jonge and G. McColl, Chem. Soc. Rev., 2015, 44, 5941
    DOI: 10.1039/C5CS00055F

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