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Issue 11, 2015
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Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

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Abstract

Microviscosity is of paramount importance in materials and bio-sciences. Fluorescence imaging using molecular rotors has emerged as a versatile tool to measure microviscosity, either using a fluorescence lifetime or a ratiometric signal of the rotor; however, only a limited number of blue-to-green-emitting fluorophores with both the lifetime and the ratiometric signal sensitivity to viscosity have been reported to date. Here we report a deep red emitting dual viscosity sensor, which allows both the ratiometric and the lifetime imaging of viscosity. We study viscosity in a range of lipid-based systems and conclude that in complex dynamic systems dual detection is preferable in order to independently verify the results of the measurements as well as perform rapid detection of changing viscosity.

Graphical abstract: Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

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Publication details

The article was received on 23 Jan 2015, accepted on 16 Feb 2015 and first published on 17 Feb 2015


Article type: Paper
DOI: 10.1039/C5CP00439J
Author version available: Download Author version (PDF)
Citation: Phys. Chem. Chem. Phys., 2015,17, 7548-7554
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    Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

    A. Vyšniauskas, M. Balaz, H. L. Anderson and M. K. Kuimova, Phys. Chem. Chem. Phys., 2015, 17, 7548
    DOI: 10.1039/C5CP00439J

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