Issue 11, 2015

The effect of an oligonucleotide on the structure of cationic DODAB vesicles

Abstract

The effect of a small single-stranded oligonucleotide (ODN) on the structure of cationic DODAB vesicles was investigated by means of differential scanning calorimetry (DSC), small angle X-ray scattering (SAXS) and electron spin resonance (ESR) spectroscopy. ODN adsorption induced coalescence of vesicles and formation of multilamellar structures with close contact between lamellae. It also increased the phase transition temperature by 10 °C but decreased transition cooperativity. The ODN rigidified and stabilized the gel phase. In the fluid phase, a simultaneous decrease of ordering close to the bilayer surface and increase in bilayer core rigidity was observed in the presence of the ODN. These effects may be due not only to electrostatic shielding of DODAB head groups but also to superficial dehydration of the bilayers. The data suggest that oligonucleotides may induce the formation of a multilamellar poorly hydrated coagel-like phase below phase transition. These effects should be taken into account when planning ODN delivery employing cationic bilayer carriers.

Graphical abstract: The effect of an oligonucleotide on the structure of cationic DODAB vesicles

Article information

Article type
Paper
Submitted
04 Dec 2014
Accepted
12 Feb 2015
First published
12 Feb 2015

Phys. Chem. Chem. Phys., 2015,17, 7498-7506

Author version available

The effect of an oligonucleotide on the structure of cationic DODAB vesicles

J. H. K. Rozenfeld, E. L. Duarte, L. R. S. Barbosa and M. T. Lamy, Phys. Chem. Chem. Phys., 2015, 17, 7498 DOI: 10.1039/C4CP05652C

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