A fluorescent G-quadruplex probe for the assay of base excision repair enzyme activity†
Abstract
A sensitive and selective assay of uracil DNA glycosylase (UDG) activity is developed by utilizing a G-quadruplex probe incorporating 2-aminopurine (2-AP), based on a novel design that excision reaction promoted by UDG triggers the formation of G-quadruplex structure with significant fluorescence enhancement of 2-AP within the probe.