Issue 69, 2015
From the journal:

Chemical Communications

DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity†

Zhen Wang,‡a   Yong Li,‡a   Lijun Li,ab   Daiqi Li,a   Yan Huang,*a   Zhou Nie*a  and  Shouzhuo Yaoa  

* Corresponding authors

a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China
E-mail: huangyan.hnu@gmail.com, niezhou.hnu@gmail.com
Fax: +86-731-88821848
Tel: +86-731-88821626

b Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, P. R. China

Abstract

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated. Based on this unique quenching effect and the DNA-mediated ScGFP/GO interaction, a label-free fluorescence method has been established for homogeneously assaying the activity and inhibition of base excision repair enzyme.

Graphical abstract: DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC04759E
Article type
Communication
Submitted
09 Jun 2015
Accepted
14 Jul 2015
First published
14 Jul 2015

Chem. Commun., 2015,51, 13373-13376

Permissions

Request permissions

DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

Z. Wang, Y. Li, L. Li, D. Li, Y. Huang, Z. Nie and S. Yao, Chem. Commun., 2015, 51, 13373 DOI: 10.1039/C5CC04759E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements