Issue 94, 2015

Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

Abstract

A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.

Graphical abstract: Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

Supplementary files

Article information

Article type
Communication
Submitted
29 Aug 2015
Accepted
27 Sep 2015
First published
29 Sep 2015

Chem. Commun., 2015,51, 16771-16773

Author version available

Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

K. D. Clark, M. M. Yamsek, O. Nacham and J. L. Anderson, Chem. Commun., 2015, 51, 16771 DOI: 10.1039/C5CC07253K

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