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Issue 94, 2015
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Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

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Abstract

A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.

Graphical abstract: Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

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Publication details

The article was received on 29 Aug 2015, accepted on 27 Sep 2015 and first published on 29 Sep 2015


Article type: Communication
DOI: 10.1039/C5CC07253K
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 16771-16773
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    Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

    K. D. Clark, M. M. Yamsek, O. Nacham and J. L. Anderson, Chem. Commun., 2015, 51, 16771
    DOI: 10.1039/C5CC07253K

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