Electrocatalytic interconversion of NADH and NAD+ by Escherichia coli flavohemoglobin

Abstract

E. coli flavohemoglobin, oriented at electrodes via amphiphilic polymyxin B, electrocatalytically interconverts NADH and NAD⁺ at its heme potentials operating as an electron transfer relay between the electrode and the protein FAD, where NADH/NAD⁺ is transformed. The results are crucial for the development of NAD⁺-dependent bioelectrodes for biosynthesis, biosensors and biofuel cells.