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Issue 57, 2015
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A doubly responsive probe for the detection of Cys4-tagged proteins

N. Kotera,a  E. Dubost,a  G. Milanole,a  E. Doris,a  E. Gravel,a  N. Arhel,b  T. Brotin,c  J.-P. Dutasta,c  J. Cochrane,c  E. Mari,ad  C. Boutin,d  E. Léonce,d  P. Berthaultd  and  B. Rousseau*a 
Author affiliations

* Corresponding authors

a iBiTec-S/SCBM, LabEx LERMIT, CEA Saclay, 91191 Gif-sur-Yvette, France
E-mail: bernard.rousseau@cea.fr

b Inserm U941, Hôpital St Louis, 1 avenue Claude Vellefaux, 75010 Paris, France

c Laboratoire de Chimie, CNRS, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France

d CEA Saclay, IRAMIS, NIMBE, UMR CEA/CNRS 3685, Laboratoire Structure et Dynamique par Résonance Magnétique, 91191 Gif-sur-Yvette, France

Abstract

Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized 129Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the 129Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.

Graphical abstract: A doubly responsive probe for the detection of Cys4-tagged proteins

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Publication details

The article was received on 08 Jun 2015, accepted on 12 Jun 2015 and first published on 12 Jun 2015


Article type: Communication
DOI: 10.1039/C5CC04721H
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 11482-11484
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    A doubly responsive probe for the detection of Cys4-tagged proteins

    N. Kotera, E. Dubost, G. Milanole, E. Doris, E. Gravel, N. Arhel, T. Brotin, J.-P. Dutasta, J. Cochrane, E. Mari, C. Boutin, E. Léonce, P. Berthault and B. Rousseau, Chem. Commun., 2015, 51, 11482
    DOI: 10.1039/C5CC04721H

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