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Issue 53, 2015
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Recent advances in the development of synthetic chemical probes for glycosidase enzymes

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Abstract

The emergence of synthetic glycoconjugates as chemical probes for the detection of glycosidase enzymes has resulted in the development of a range of useful chemical tools with applications in glycobiology, biotechnology, medical and industrial research. Critical to the function of these probes is the preparation of substrates containing a glycosidic linkage that when activated by a specific enzyme or group of enzymes, irreversibly releases a reporter molecule that can be detected. Starting from the earliest examples of colourimetric probes, increasingly sensitive and sophisticated substrates have been reported. In this review we present an overview of the recent advances in this field, covering an array of strategies including chromogenic and fluorogenic substrates, lanthanide complexes, gels and nanoparticles. The applications of these substrates for the detection of various glycosidases and the scope and limitations for each approach are discussed.

Graphical abstract: Recent advances in the development of synthetic chemical probes for glycosidase enzymes

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Publication details

The article was received on 03 Apr 2015, accepted on 29 May 2015 and first published on 02 Jun 2015


Article type: Feature Article
DOI: 10.1039/C5CC02793D
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 10576-10588
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    Recent advances in the development of synthetic chemical probes for glycosidase enzymes

    H. M. Burke, T. Gunnlaugsson and E. M. Scanlan, Chem. Commun., 2015, 51, 10576
    DOI: 10.1039/C5CC02793D

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