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Issue 49, 2015
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A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

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Abstract

An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL−1, and the limit of detection for TdT was 0.25 U mL−1.

Graphical abstract: A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(iii)-based i-motif probe

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Publication details

The article was received on 03 Apr 2015, accepted on 17 May 2015 and first published on 18 May 2015


Article type: Communication
DOI: 10.1039/C5CC02790J
Citation: Chem. Commun., 2015,51, 9953-9956
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    A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe

    L. Lu, M. Wang, L. Liu, C. Wong, C. Leung and D. Ma, Chem. Commun., 2015, 51, 9953
    DOI: 10.1039/C5CC02790J

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