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Issue 41, 2015
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A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels

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Abstract

A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3–7-fold) for three hydrolases (esterase, lipase, cellulase). The screening principle is based on coupled enzymatic reaction using glucose derivatives which yield upon hydrolysis a fluorescent-hydrogel-layer on the surface of E. coli cells.

Graphical abstract: A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels

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Publication details

The article was received on 02 Mar 2015, accepted on 16 Apr 2015 and first published on 16 Apr 2015


Article type: Communication
DOI: 10.1039/C5CC01791B
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 8679-8682
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    A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels

    N. Lülsdorf, C. Pitzler, M. Biggel, R. Martinez, L. Vojcic and U. Schwaneberg, Chem. Commun., 2015, 51, 8679
    DOI: 10.1039/C5CC01791B

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