Jump to main content
Jump to site search

Issue 48, 2015
Previous Article Next Article

Directed evolution 2.0: improving and deciphering enzyme properties

Author affiliations

Abstract

Directed evolution has matured to a routinely applied algorithm to tailor enzyme properties to meet the demands in various applications. In order to free directed enzyme evolution from methodological restraints and to efficiently explore its potential, many different strategies have been used in directed evolution campaigns. Analysis of directed evolution campaigns reveals that traditional approaches, in which several iterative rounds of diversity generation and screening are performed, are gradually replaced by strategies which require less time, less screening efforts, and generate a molecular understanding of the targeted properties. In this review, conceptual advances in knowledge generating directed evolution strategies are summarized, compared to each other and to traditional directed evolution strategies. Finally, a ‘KnowVolution’ (knowledge gaining directed evolution) termed strategy is proposed.

Graphical abstract: Directed evolution 2.0: improving and deciphering enzyme properties

Back to tab navigation

Publication details

The article was received on 22 Feb 2015, accepted on 02 Apr 2015 and first published on 02 Apr 2015


Article type: Feature Article
DOI: 10.1039/C5CC01594D
Citation: Chem. Commun., 2015,51, 9760-9772
  •   Request permissions

    Directed evolution 2.0: improving and deciphering enzyme properties

    F. Cheng, L. Zhu and U. Schwaneberg, Chem. Commun., 2015, 51, 9760
    DOI: 10.1039/C5CC01594D

Search articles by author

Spotlight

Advertisements