Issue 13, 2015

Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases

Abstract

We describe a nucleic acid testing (NAT) platform for infectious disease diagnostics at the point-of-care, using surface acoustic waves (SAW) to perform a multiplexed loop-mediated isothermal amplification (LAMP) test for sexually transmitted diseases. The ultrasonic actuation not only enables faster NAT reactions but also provides a route towards integrating low-cost, low-power molecular diagnostics into disposable sensors.

Graphical abstract: Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases

Supplementary files

Article information

Article type
Communication
Submitted
23 Oct 2014
Accepted
23 Dec 2014
First published
23 Dec 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 2589-2592

Author version available

Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases

G. Xu, R. N. Gunson, J. M. Cooper and J. Reboud, Chem. Commun., 2015, 51, 2589 DOI: 10.1039/C4CC08389J

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