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Issue 25, 2015
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A simple bioluminescent method for measuring D-amino acid oxidase activity

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Abstract

D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.

Graphical abstract: A simple bioluminescent method for measuring d-amino acid oxidase activity

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Publication details

The article was received on 14 Oct 2014, accepted on 30 Oct 2014 and first published on 03 Nov 2014


Article type: Communication
DOI: 10.1039/C4CC08145E
Citation: Chem. Commun., 2015,51, 5425-5428
  • Open access: Creative Commons BY license
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    A simple bioluminescent method for measuring D-amino acid oxidase activity

    T. S. Bailey, M. T. Donor, S. P. Naughton and M. D. Pluth, Chem. Commun., 2015, 51, 5425
    DOI: 10.1039/C4CC08145E

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