Jump to main content
Jump to site search

Issue 13, 2015
Previous Article Next Article

A high-throughput screening method for determining the substrate scope of nitrilases

Author affiliations

Abstract

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

Graphical abstract: A high-throughput screening method for determining the substrate scope of nitrilases

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Aug 2014, accepted on 19 Dec 2014 and first published on 22 Dec 2014


Article type: Communication
DOI: 10.1039/C4CC06021K
Citation: Chem. Commun., 2015,51, 2660-2662
  • Open access: Creative Commons BY license
  •   Request permissions

    A high-throughput screening method for determining the substrate scope of nitrilases

    G. W. Black, N. L. Brown, J. J. B. Perry, P. D. Randall, G. Turnbull and M. Zhang, Chem. Commun., 2015, 51, 2660
    DOI: 10.1039/C4CC06021K

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements