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Issue 5, 2015
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MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

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Abstract

Multi-component nucleotide acid enzymes (MNAzymes) derived from RNase-mimic DNAzymes have potential as simple and accurate DNA detectors. To enhance the MNAzyme activity under multiple-turnover conditions, a cationic comb-type copolymer, PLL-g-Dex, that facilitates hybridization and strand exchange reactions of DNA was utilized. The copolymer increased the MNAzyme reaction rate by 200 times, allowing target DNA detection at picomolar concentrations at physiological temperature.

Graphical abstract: MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

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Publication details

The article was received on 22 Dec 2014, accepted on 22 Feb 2015 and first published on 11 Mar 2015


Article type: Paper
DOI: 10.1039/C4BM00449C
Citation: Biomater. Sci., 2015,3, 716-720
  • Open access: Creative Commons BY license
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    MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

    J. Gao, N. Shimada and A. Maruyama, Biomater. Sci., 2015, 3, 716
    DOI: 10.1039/C4BM00449C

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