Issue 5, 2015

MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

Abstract

Multi-component nucleotide acid enzymes (MNAzymes) derived from RNase-mimic DNAzymes have potential as simple and accurate DNA detectors. To enhance the MNAzyme activity under multiple-turnover conditions, a cationic comb-type copolymer, PLL-g-Dex, that facilitates hybridization and strand exchange reactions of DNA was utilized. The copolymer increased the MNAzyme reaction rate by 200 times, allowing target DNA detection at picomolar concentrations at physiological temperature.

Graphical abstract: MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

Supplementary files

Article information

Article type
Paper
Submitted
22 Dec 2014
Accepted
22 Feb 2015
First published
11 Mar 2015
This article is Open Access
Creative Commons BY license

Biomater. Sci., 2015,3, 716-720

Author version available

MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

J. Gao, N. Shimada and A. Maruyama, Biomater. Sci., 2015, 3, 716 DOI: 10.1039/C4BM00449C

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