Issue 9, 2015

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

Abstract

Mammalian cell culture is the starting point in many research studies focusing on biomedical applications. However, researchers have little control over the standardized cell microenvironment parameters. Here a modular ECM-mimicking surface coating for cell culture environment is designed. This substrate is a new and versatile thin film obtained by spin-coating of concentrated gelatin crosslinked by transglutaminase. It can be modified with respect to the biochemical and biophysical needs of the final cell destination, i.e. it delivers loaded multi-growth factors and serum components and allows for cell culture in a serum-free culture medium. Also, a well-known cell behavior modulator, the substrate stiffness, is controlled exogenously by addition of nanoparticles. In addition to growth factors, antimicrobial agents such as natural peptides are added to the substrate for limiting the repeated addition of antimicrobial agents to the culture medium and to prevent the increase of resistant bacterial strains in the culture environment. Finally, this substrate contains simultaneously ECM components, growth factors, stiffening elements and antimicrobial agents. It provides a favorable microenvironment and sterile conditions. It is a free-of-maintenance system, as cells will grow without addition of serum or antimicrobial cocktails. This low cost and easy-to-use substrate could emerge as a new standard for cell culture.

Graphical abstract: Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

Supplementary files

Article information

Article type
Paper
Submitted
04 Jun 2015
Accepted
14 Jun 2015
First published
01 Jul 2015

Biomater. Sci., 2015,3, 1302-1311

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

J. Barthes, N. E. Vrana, H. Özçelik, R. Gahoual, Y. N. François, J. Bacharouche, G. Francius, J. Hemmerlé, M. Metz-Boutigue, P. Schaaf and P. Lavalle, Biomater. Sci., 2015, 3, 1302 DOI: 10.1039/C5BM00172B

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