Issue 12, 2015

Electrochemical behaviors and simultaneous determination of guanine and adenine based on highly ordered Pd-nanowire arrays-modified glassy carbon electrode

Abstract

A novel electrochemical sensor for the simultaneous detection of guanine (G) and adenine (A) was developed on a Pd nanowire arrays-modified carbon glassy electrode (Pd NWAs). The Pd NWAs were obtained via the direct electrode position of Pd on a glassy carbon electrode within the pores of an anodized aluminum oxide membrane. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray (EDS) techniques were used to examine the Pd NWAs structures. The Pd nanowire arrays have high electrochemically active surfaces, and this modified electrode exhibits excellent and persistent electro-oxidation behavior toward G and A. The Pd NWAs modified electrodes significantly increases oxidation peak currents but reduces the peaks potential of G and A. For the Pd NWAs-modified electrode, the peak potentials of G and A are negatively shifted to 0.69 and 0.97 V, respectively. Differential pulse voltammetry (DPV) was proposed for the simultaneous determination of G and A. Under the selected conditions, the oxidation peak currents were proportional to guanine and adenine, ranging from 2 μM to 200 μM and 4 μM to 200 μM with detection limits at 0.7 μM and 1 μM, respectively. Simultaneously, the proposed method was successfully applied for the determination of guanine and adenine in denatured DNA samples with satisfactory results.

Graphical abstract: Electrochemical behaviors and simultaneous determination of guanine and adenine based on highly ordered Pd-nanowire arrays-modified glassy carbon electrode

Supplementary files

Article information

Article type
Paper
Submitted
09 Mar 2015
Accepted
29 Apr 2015
First published
22 May 2015

Anal. Methods, 2015,7, 4988-4994

Electrochemical behaviors and simultaneous determination of guanine and adenine based on highly ordered Pd-nanowire arrays-modified glassy carbon electrode

M. Zhang, F. Gan and F. Cheng, Anal. Methods, 2015, 7, 4988 DOI: 10.1039/C5AY00608B

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