Jump to main content
Jump to site search

Issue 5, 2015
Previous Article Next Article

Amino acid composition of human scalp hair as a biometric classifier and investigative lead

Author affiliations

Abstract

Hair shaft analysis is becoming increasingly important in several applications of forensic science. Keratin is the key component of human scalp hair and is composed of all 21 known amino acids, albeit in very different proportions. The method for the amino acid determination included protein acid hydrolysis followed by trimethylsilyl (TMS) derivatization of the amino acids, and the subsequent quantitation using gas chromatography/mass spectrometry (GC/MS). The amino acid composition of scalp hair of 64 Jordanian subjects (33 males and 31 females) with ages ranging from 1 to 77 years have been analyzed. Statistical comparisons between classification groups were based on the abundance of 14 abundant and acid-resistant amino acids, and included classification of hairs with a fuzzy rule building system (FuRES). Using leave-one-individual-out cross-validation, the FuRES classification rate was 94% for sex, 83% for age group, and 61% for the region of origin. For predicting sex from amino acid concentration in hair, the essential amino acids Phe and Thr gave the most significant differences with respect to their F statistic (i.e., ratio of between-group to within-group variation), so they are the most discriminating for sex. Based on the same hair analyses, the non-essential amino acids Gly and Ala provide the largest loading scores classifying the subjects into two arbitrary age groups, <49 and >49 years. For region of origin classification, the amino acids Cys and Tyr had the highest loading scores in the classification rules and were therefore most discriminating. The techniques developed through this paper could complement the current methods of hair analysis, which include physical examinations and genomic or mitochondrial DNA analysis.

Graphical abstract: Amino acid composition of human scalp hair as a biometric classifier and investigative lead

Back to tab navigation

Publication details

The article was received on 30 Oct 2014, accepted on 25 Nov 2014 and first published on 25 Nov 2014


Article type: Paper
DOI: 10.1039/C4AY02588A
Author version available: Download Author version (PDF)
Citation: Anal. Methods, 2015,7, 1707-1718
  •   Request permissions

    Amino acid composition of human scalp hair as a biometric classifier and investigative lead

    A. H. B. Rashaid, P. B. Harrington and G. P. Jackson, Anal. Methods, 2015, 7, 1707
    DOI: 10.1039/C4AY02588A

Search articles by author

Spotlight

Advertisements