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Issue 10, 2015
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DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

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Abstract

In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.

Graphical abstract: DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

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Publication details

The article was received on 15 Jan 2015, accepted on 25 Feb 2015 and first published on 25 Feb 2015


Article type: Paper
DOI: 10.1039/C5AN00093A
Citation: Analyst, 2015,140, 3422-3430
  • Open access: Creative Commons BY license
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    DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

    P. Shah, S. W. Choi, H. Kim, S. K. Cho, P. W. Thulstrup, M. J. Bjerrum, Y. Bhang, J. C. Ahn and S. W. Yang, Analyst, 2015, 140, 3422
    DOI: 10.1039/C5AN00093A

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