Jump to main content
Jump to site search

Issue 14, 2015
Previous Article Next Article

Metal-enhanced fluorescence and FRET on nanohole arrays excited at angled incidence

Author affiliations

Abstract

The influence of experimental parameters on the performance of plasmonic sensors is of great importance in analytical sciences. The plasmon coupling conditions (angle of incidence, metal composition, laser frequency and excitation/emission properties of fluorophores) were thus investigated for surface plasmon-enhanced fluorescence on metallic nanohole arrays. Optimal fluorescence enhancements were achieved when the plasmon resonance, the excitation laser and the fluorophore's excitation wavelengths were matched. The enhancement of the acceptor emission of a rhodamine 6G(Rh6G)-Quasar670™ FRET pair was achieved on the nanohole arrays by tuning the plasmon wavelength with the maximal overlap of the donor's emission and acceptor excitation. Silver nanohole arrays achieved larger fluorescence enhancement than gold nanohole arrays at 532 nm, while gold nanohole arrays led to larger fluorescence enhancement at 635 nm. These results demonstrate the importance of tuning the plasmon coupling conditions for surface plasmon-enhanced fluorescence sensing.

Graphical abstract: Metal-enhanced fluorescence and FRET on nanohole arrays excited at angled incidence

Back to tab navigation

Publication details

The article was received on 09 Dec 2014, accepted on 31 Jan 2015 and first published on 02 Feb 2015


Article type: Communication
DOI: 10.1039/C4AN02257B
Citation: Analyst, 2015,140, 4792-4798
  •   Request permissions

    Metal-enhanced fluorescence and FRET on nanohole arrays excited at angled incidence

    H.-P. Poirier-Richard, M. Couture, T. Brule and J.-F. Masson, Analyst, 2015, 140, 4792
    DOI: 10.1039/C4AN02257B

Search articles by author

Spotlight

Advertisements