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Issue 4, 2015
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Direct detection of microRNA based on plasmon hybridization of nanoparticle dimers

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Abstract

MicroRNAs (miRNA) are important for regulating a range of biochemical pathways. Abnormal levels of miRNA in cells or secreted into biological fluids have been identified in diseases. MiRNA can therefore be potential biomarkers for early disease diagnosis; however their detection and quantification are challenging. Herein we apply the sensing platform of discrete actuatable dimers for the detection of human miR-210 (hsa-miR-210-3p). The detection signal is a spectral blue shift in the hybridized plasmon mode as monitored by single-nanostructure spectroscopy. We investigate the specificity and detection limit of the platform and quantify miR-210 levels in RNA extracts of cells cultured under different oxygen tensions. In addition we demonstrate the feasibility of detection in complex media by examining miR-210 secreted in cell media. This sensing platform may be developed as a bioanalytical tool for validating miRNA profiles of biological fluids.

Graphical abstract: Direct detection of microRNA based on plasmon hybridization of nanoparticle dimers

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Publication details

The article was received on 27 Nov 2014, accepted on 03 Dec 2014 and first published on 03 Dec 2014


Article type: Paper
DOI: 10.1039/C4AN02189D
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 1140-1148
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    Direct detection of microRNA based on plasmon hybridization of nanoparticle dimers

    Y. Wang, E. MacLachlan, B. K. Nguyen, G. Fu, C. Peng and J. I. L. Chen, Analyst, 2015, 140, 1140
    DOI: 10.1039/C4AN02189D

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