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Issue 14, 2015
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Superposition of an AC field improves the discrimination between peptides in nanopore analysis

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Abstract

In standard nanopore analysis a constant DC voltage is used to electrophoretically drive small molecules and peptides towards a pore. Superposition of an AC voltage at particular frequencies causes molecules to oscillate as they approach the pore which can alter the event parameters, the blockade current (I) and blockade time (T). Four peptides with similar structures were studied. Alpha-helical peptides A10 (FmocDDA10KK), A14, A18 and retro-inverso A10. It was shown that the ratio of translocations to bumping events could be manipulated by a combination of AC voltages and frequencies. In particular, A10 could be studied without interference from retro-inverso A10. Similarly, a large, intrinsically disordered protein of 140 amino acids, α-synuclein, which translocates the pore readily in a DC field could be prevented from doing so by application of an AC field of 200 mV at 100 MHz.

Graphical abstract: Superposition of an AC field improves the discrimination between peptides in nanopore analysis

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Publication details

The article was received on 26 Nov 2014, accepted on 14 Feb 2015 and first published on 16 Feb 2015


Article type: Paper
DOI: 10.1039/C4AN02180K
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 4813-4819
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    Superposition of an AC field improves the discrimination between peptides in nanopore analysis

    E. Jakova and J. S. Lee, Analyst, 2015, 140, 4813
    DOI: 10.1039/C4AN02180K

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