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Issue 4, 2015
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A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

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Abstract

A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure from open to closed states and produces a FRET signal in response to in vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 mRNA as a pre-fixed target mRNA model and Hes-1 mRNA as a model expressed inside a living cell. These results confirm the feasibility of using the FRET-based DNA-NT for imaging analysis of target mRNA.

Graphical abstract: A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

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The article was received on 10 Nov 2014, accepted on 10 Dec 2014 and first published on 10 Dec 2014


Article type: Communication
DOI: 10.1039/C4AN02064B
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 999-1003
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    A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

    H. Funabashi, H. Shigeto, K. Nakatsuka and A. Kuroda, Analyst, 2015, 140, 999
    DOI: 10.1039/C4AN02064B

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