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Issue 7, 2015
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Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

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Abstract

A recent upgrade to the optics configuration of a thermal source FTIR microscope equipped with a focal plane array detector has enabled rapid acquisition of high magnification spectrochemical images, in transmission, with an effective geometric pixel size of ∼1 × 1 μm2 at the sample plane. Examples, including standard imaging targets for scale and accuracy, as well as biomedical tissues and microorganisms, have been imaged with the new system and contrasted with data acquired at normal magnification and with a high magnification multi-beam synchrotron instrument. With this optics upgrade, one can now conduct rapid biodiagnostic ex vivo tissue imaging in-house, with images collected over larger areas, in less time (minutes) and with comparable quality and resolution to the best synchrotron source FTIR imaging capabilities.

Graphical abstract: Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

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Publication details

The article was received on 30 Oct 2014, accepted on 07 Jan 2015 and first published on 07 Jan 2015


Article type: Paper
DOI: 10.1039/C4AN01982B
Citation: Analyst, 2015,140, 2493-2503
  • Open access: Creative Commons BY license
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    Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

    C. R. Findlay, R. Wiens, M. Rak, J. Sedlmair, C. J. Hirschmugl, J. Morrison, C. J. Mundy, M. Kansiz and K. M. Gough, Analyst, 2015, 140, 2493
    DOI: 10.1039/C4AN01982B

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