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Issue 1, 2015
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An adaptable stage perfusion incubator for the controlled cultivation of C2C12 myoblasts

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Abstract

Here we present a stage perfusion incubation system that allows for the cultivation of mammalian cells within PDMS microfluidic devices for long-term microscopic examination and analysis. The custom-built stage perfusion incubator is adaptable to any xy microscope stage and is enabled for temperature, gas and humidity control as well as equipped with chip and tubing holder. The applied double-layered microfluidic chip allows the predetermined positioning and concentration of cells while the gas permeable PDMS material facilitates pH control via CO2 levels throughout the chip. We demonstrate the functionality of this system by culturing C2C12 murine myoblasts in buffer free medium within its confines for up to 26 hours. We moreover demonstrated the system's compatibility with various chip configurations, other cells lines (HEK-293 cells) and for longer-term culturing. The cost-efficient system are applicable for any type of PDMS-based cell culture system. Detailed technical drawings and specification to reproduce this perfusion incubation system is provided in the ESI.

Graphical abstract: An adaptable stage perfusion incubator for the controlled cultivation of C2C12 myoblasts

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Publication details

The article was received on 28 Sep 2014, accepted on 23 Oct 2014 and first published on 24 Oct 2014


Article type: Paper
DOI: 10.1039/C4AN01758G
Citation: Analyst, 2015,140, 127-133
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    An adaptable stage perfusion incubator for the controlled cultivation of C2C12 myoblasts

    F. Kurth, A. Franco-Obregón, C. A. Bärtschi and Petra. S. Dittrich, Analyst, 2015, 140, 127
    DOI: 10.1039/C4AN01758G

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