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Issue 5, 2015
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Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

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Abstract

DNA-stabilized fluorescent silver nanoclusters (AgNC DNA) are a new class of fluorophore that are formed by sequence specific interactions between silver and single-stranded DNA. By incorporating both target-binding and fluorescent-reporting sequences into a single synthetic DNA oligomer, AgNC DNA probes eliminate the need to conjugate dye or quencher molecules. In this study, we modify a AgNC DNA probe to demonstrate single-color multiplexed detection of DNA targets. We show that appending different lengths of poly-dT to the probe sequences tunes the electrophoretic mobility of AgNC DNA probes without affecting their fluorescence spectra. We use this to introduce a set of AgNC DNA probes selective for Hepatitis A, B and C target sequences that can be processed together in a simple, single-step protocol and distinguished with a resolution of 3.47 and signal to noise ratio of 17.23 in under 10 seconds by microfluidic capillary electrophoresis.

Graphical abstract: Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

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Publication details

The article was received on 24 Sep 2014, accepted on 19 Dec 2014 and first published on 09 Jan 2015


Article type: Paper
DOI: 10.1039/C4AN01735H
Author version available: Download Author version (PDF)
Citation: Analyst, 2015,140, 1609-1615
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    Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

    J. T. Del Bonis-O'Donnell, D. K. Fygenson and S. Pennathur, Analyst, 2015, 140, 1609
    DOI: 10.1039/C4AN01735H

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